Soluble HLA-DQ2 expressed in S2 cells copurifies with a high affinity insect cell derived protein
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چکیده
منابع مشابه
Design of gliadin peptide analogues with low affinity for the celiac disease associated HLA-DQ2 protein.
The HLA-DQ2.5 receptors bind gluten-derived peptides and present them to the T cells in the intestinal mucosa thus inducing the development of immune responses typical of the celiac disease. On the basis of the X-ray structure of the domain of HLA-DQ2.5 bound to the DQ2.5-glia-α1a epitope, fifteen peptides were designed with the aim of lowering the epitope binding affinity, thus reducing the au...
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We here describe that soluble HLA-DQ2 (sDQ2)molecules, when expressed in Drosophila melanogaster S2insect cells without a covalently tethered peptide, associatetightly with the D. melanogaster calcium binding proteinDCB-45. The interaction between the proteins is stable in S2cell culture and during affinity purification, which is done athigh salt concentrations and pH 11...
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one of the emerging therapeutic strategies for targeted treatment of most cancers is the use of immunotoxins which are fusion proteins consisted of a targeting and a toxic moieties. we previously showed that the recombinant a254-gmcsf fusion protein selectively kills acute myeloblastic leukemia cells which harbor a large number of granulocyte-macrophage colony stimulating factor (gmcsf) recepto...
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Introduction: Recognition of human leukocyte antigens (HLA) is of importance for hematopoietic stem cell transplantation. Any HLA-mismatches between the donor and recipient can cause graft rejection or other complications. In HLA-typing experiments, usage of HLA-known reference cells accompany with HLA-unknown samples is obligatory. Some international centers represent these cells with high e...
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ژورنال
عنوان ژورنال: Immunogenetics
سال: 2008
ISSN: 0093-7711,1432-1211
DOI: 10.1007/s00251-008-0338-7